PAM-2500 Chlorophyll Fluorometer

Brand: Heinz Walz GmbH
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Brand: Heinz Walz GmbH

Product Code: PAM2500

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Specifications: (see all)

Measured / controlled parameters

Chlorophyll fluorescence (Ft, Fm, Fm’, Fo, Fo’), PSII quantum yield (Y(II)), electron transport rate (ETR), quenching parameters (NPQ, qP), fluorescence induction and relaxation kinetics, sample temperature, light flux, actinic light intensity.

Measurement / analysis range

Actinic light intensity – 0 to 25 000 µmol photons m⁻² s⁻¹; measuring-light frequency – 10 to 200 000 Hz; pulse duration – 1 µs.

Measurement accuracy / precision

High sensitivity to modulated signals ± 1 % of scale; noise < 0.1 % of signal.

Analysis method / operating principle

Pulse-Amplitude-Modulation (PAM) method – modulated measuring light excites chlorophyll, fluorescence is detected with high selectivity even under strong ambient light.

Analysis / measurement time

Single measurement (SAT pulse) ≈ 1–2 s; fast kinetics with 10 µs resolution; long-term curves up to several hours.

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The PAM-2500 Portable Chlorophyll Fluorometer by Heinz Walz GmbH (Germany) continues the legacy of the PAM-2000/2100 series, integrating modern electronics and optical systems for high-precision chlorophyll fluorescence measurements and PSII performance evaluation.

The device utilizes pulse-modulated measuring light (1 µs pulses, 10–200 kHz) and actinic LED sources at 455 nm (blue), 630 nm (red), and 750 nm (far-red). It achieves exceptional signal sensitivity even under full sunlight, while allowing fluorescence analysis from basic to advanced kinetic levels — from Kautsky induction to fast fluorescence decay.

With the PamWin-3 software, users can perform automated measurement sequences via Script Files, manage saturation pulse analysis, and obtain reliable light and induction curves. The instrument supports both desktop and ultra-mobile touchscreen PCs (UMPCs) for convenient field operation.

Compact (23 × 10.5 × 10.5 cm) yet powerful, the PAM-2500 is perfectly suited for plant ecophysiology, stress screening, and fundamental research involving leaves, chloroplast suspensions, algae, and cyanobacteria.

Tags: Soil and plant analysis

Soil and plant analysis

Measured / controlled parameters

Measurement / analysis range

Actinic light intensity – 0 to 25 000 µmol photons m⁻² s⁻¹; measuring-light frequency – 10 to 200 000 Hz; pulse duration – 1 µs.

Measurement accuracy / precision

High sensitivity to modulated signals ± 1 % of scale; noise < 0.1 % of signal.

Analysis method / operating principle

Pulse-Amplitude-Modulation (PAM) method – modulated measuring light excites chlorophyll, fluorescence is detected with high selectivity even under strong ambient light.

Analysis / measurement time

Single measurement (SAT pulse) ≈ 1–2 s; fast kinetics with 10 µs resolution; long-term curves up to several hours.

Sample volume / sample volume range

Leaf area ≈ 1 cm² (with 2030-B clip) or cuvette volume 1–3 mL (KS-2500 model).

Applicable media

Plant leaves, chloroplast suspensions, algal and cyanobacterial cultures.

Sensor / electrode / probe / indicator type

High-sensitivity photodiode (fluorescence detector); multi-color LED sources (red 630 nm, blue 455 nm, far-red 750 nm).

Number of channels / measurement points

Single optical channel with connectable leaf or cuvette sensors.

Temperature compensation

Built-in temperature sensor with automatic temperature compensation for fluorescence response.

Number of calibration points / calibration method

Light-intensity and detector-gain calibration via PamWin-3; automatic self-calibration available.

Housing / chamber / tip material

Aluminum housing, fiber-optic probe with protective sheath, leaf clip of stainless steel and polymer.

Design and features

Compact unit (23 × 10.5 × 10.5 cm); all optical and electronic modules in one housing; detachable fiber optic cable.

Lighting / climate control system

Actinic LED sources (blue, red, far-red) and measuring light 630 nm pulsed up to 200 kHz.

Number of programmable cycles / automatic modes

Automated protocols via Script Files in PamWin-3 for reproducible measurements.

Interfaces and outputs

USB 2.0 / RS-232 for PC connection; fiber-optic and accessory ports.

Software / PC compatibility

PamWin-3 (Heinz Walz) for Windows – quenching analysis, light curves, fast and slow kinetics.

Memory / data storage functions

Data storage on PC with automatic protocol generation in PamWin-3.

Operating conditions

Operating temperature 0…+45 °C; humidity up to 90 % (non-condensing).

Power supply

Powered via AC adapter (100–240 V, 50/60 Hz) or portable UMPC through USB.

Battery life

Up to 8 hours when operated from UMPC or external battery.

Dimensions

23 × 10.5 × 10.5 cm.

Weight

Approx. 1.5 kg (including cable).

Scope of supply

PAM-2500 fluorometer, 2030-B leaf clip, KS-2500 cuvette with stirrer, optional UMPC, PamWin-3 software, cables, manual.

Certificates and compliance with standards

CE, ISO 9001, GLP compliant; manufactured by Heinz Walz GmbH.

Country of origin

Germany

Storage conditions

Store dry at +5…+40 °C, protect from dust and moisture.

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